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months, he is experiencing weaknessand loss of appetite. Zeltser D, Rosansky S, Van Rensberg H, Verbalis JG, Smith N; ConivaptanStudy Group. Differential centrifuga-tion of liver tissue gently homogenized in 0.25M sucrose (soas to preserve mitochondria intact) can yield other fractionscontaining xenobiotic-metabolizing enzymes; 18,000g for20min will pull down intact heavy and light mitochondriainto a pellet, and microsomes require 105,000g for 60min tosediment, leaving the cytosolic (soluble) fraction as a super-natant

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